- Why assay is performed?
- What is purity threshold in HPLC?
- What is the basic principle of chromatography?
- Does higher RF mean more polar?
- What is Rf value?
- What are the different types of impurities?
- Why is RF less than 1?
- How does chromatography show purity?
- Why is RF value important?
- What is the point of chromatography?
- Why assay is more than 100?
- Why PDA detector is used in HPLC?
- What does higher RF value mean?
- What is difference between purity and potency?
- What is the difference between chromatographic purity and related substances?
- What is peak purity?
- What is assay and purity?
- What is threshold in HPLC?
- Where is chromatography used in real life?
- Is assay and purity the same?
- What are related substances?
Why assay is performed?
An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte)..
What is purity threshold in HPLC?
Peak purity is a comparison of the reference standard to the API in the sample stressed by ‘forced degradation (thus specificity). In essence you are showing that no impurity (related substance) is eluting underneath the main API peak in HPLC.
What is the basic principle of chromatography?
Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.
Does higher RF mean more polar?
In general, the adsorptivity of compounds increases with increased polarity (i.e. the more polar the compound then the stronger it binds to the adsorbent). … Non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value).
What is Rf value?
The Rf value The retention factor, or Rf, is defined as the distance traveled by the compound divided by the distance traveled by the solvent. For example, if a compound travels 2.1 cm and the solvent front travels 2.8 cm, the Rf is. 0.75: The Rf for a compound is a constant from one experiment to the next only if the.
What are the different types of impurities?
Impurities can be classified into the following categories: Organic Impurities (Process And Drug-Related) Inorganic Impurities Residual Solvents Organic Impurities : Organic impurities can arise during the manufacturing process and/or storage of the new drug substance.
Why is RF less than 1?
By definition, Rf values are always less than 1. An Rf value of 1 or too close to it means that the spot and the solvent front travel close together and is therefore unreliable. This happens when the eluting solvent is too polar for the sample.
How does chromatography show purity?
Using chromatography to check purity A chromatogram produced by paper chromatography or thin layer chromatography (TLC) can be used to distinguish between pure and impure substances: a pure substance produces one spot on the chromatogram. an impure substance produces two or more spots.
Why is RF value important?
Rf value stands for the retardation factor value. It tells us how far the unknown pigment traveled in relation to the distance the solvent traveled. The Rf value is useful for scientists because it allows scientists to identify the pigment by comparing its Rf value to that of a known standard.
What is the point of chromatography?
‘Chromatography’ is an analytical technique commonly used for separating a mixture of chemical substances into its individual components, so that the individual components can be thoroughly analyzed.
Why assay is more than 100?
There is a simple reason to have the purity greater than 100% for this compound. If the substance was exposed to a dry environment for several hours, a small amount of the water of hydration could be lost, causing the calculation to have a higher purity.
Why PDA detector is used in HPLC?
Diode-Array Detection can be used to identify unknown peaks observed in chromatography. Diode-Array Detection (DAD) or Photodiode-Array Detection (PDA) is an analytical technique that can be used to determine the purity of an analyte or related impurity peak eluting during an HPLC separation.
What does higher RF value mean?
distance traveled by substanceRf = distance traveled by substance/distance traveled by solvent front. A high Rf (Ie 0.92) would refer to a substance that is very non-polar. Ie that substance moved a 92% of the entire distance the solvent traveled. A low Rf value (0.10) would refer to a substance that is very polar.
What is difference between purity and potency?
-Potency is a measure of drug activity expressed in terms of the amount required to produce an effect of given intensity. -Purity is a measure of the amount of API present in a sample compared to those of related substances, impurities, residual solvents, etc.
What is the difference between chromatographic purity and related substances?
I my place of work we use the term “chromatographic purity method” when the results are reported as %Area of the various peaks. Where as the term “related substances method” is used when one uses a diluted standard to quantify the known and unknown impurities in the sample and report the results in %w/w terms.
What is peak purity?
Peak Purity is an analysis of absorbance spectra across the peak to determine if they are all similar or there are differences. If there are spectral differences, it implies there are two or more compounds eluting in that chromatographic peak each being spectrally different.
What is assay and purity?
According to the “WikiAnswers” website, an “assay” method provides an experimentally determined value for the content or potency of an analyte in a sample. In contrast, a “purity” methods provides an accurate quantitative statement of all impurities in a pharmaceutical sample.
What is threshold in HPLC?
Threshold. ▪ Specifies the liftoff and touchdown values (minimum rate of change of the detector signal) for peak detection.
Where is chromatography used in real life?
Chromatography is also used to help catch criminals. In line with programmes like CSI, gas chromatography is used to analyse blood and cloth samples, helping to identify criminals and bring them to justice. It’s clear to see that chromatography is an unsung hero when it comes to keeping you healthy and safe everyday.
Is assay and purity the same?
The main difference between assay and purity is that an assay is the determination of one of the main component in a sample whereas purity is the determination of impurities in a sample. … Assay and purity are two types of measurements used to determine the components of a sample.
What are related substances?
Related Substances— Related substances are structurally related to a drug substance. These substances may be identified or unidentified degradation products or impurities arising from a manufacturing process or during storage of a material.